Trânsito gastrintestinal em camundongos c57 durante parasitose por strongyloides venezuelensis

Abstract

Strongyloidiasis is a neglected parasitic infection, usually asymptomatic and related to precarious hygiene conditions. The objective of this study was to evaluate the gastrointestinal transit of C57BL / 6 mice infected by Strongyloides venezuelensis using the Alternating Current Biosusceptometry (BAC) technique. Adult male mice were randomly divided into two groups: Control (n = 5) and Infected (n = 6) who received a parasitic load of 1500 L3 larvae. During 14 days post infection (dpi), the counting of eggs per gram of feces (OPG) was performed confirming the infection of the animals. At 7 ° and 14 ° dpi, both groups were fasted for 12 hours and then received ration with magnetic marker to record the value of their initial intensity. The percentage of Initial Excretion (first peak of magnetic intensity in the faeces relative to that of the ingested ration), percentage of Final Excretion (total excretion of feces in 10h in relation to the initial intensity of the ingested ration) was determined using the BAC technique: (TTOA), demonstrating the period that the marked ration took to transit from the mouth to the anus, the Average Gastrin-Transit Time (TMTG) over 10h. In the results the OPG showed the peak of infection in the 8dpi. The percentage of initial excretion did not differ between the control group (4.1% ± 1.3%) and the infected group in the 7dpi (4.2% ± 2.2%) and 14dpi (2.0% ± 0, 4%), demonstrating the validity of the test. The percentage of the Final Excretion showed a higher retention of the material ingested in the 7dpi Infected group (67.6% ± 26.5%) compared to Control (33.1% ± 16.2%) (p <0.05) and to 14dpi (23.3% ± 8.8%) (p <0.01). The TTOA was slowed in the Infected group 7dpi (306.4 min ± 80.4 min) compared to Control (137.1 min ± 37.3 min) (p <0.0001) and the 14dpi group (185.5 min ± 38.3 min) (p <0.01). The TMTG of the 14 dpi group (425.8 min ± 33.1min) showed an increase compared to the 7dpi group (309.0 min ± 66.9min) (p <0.05) and in relation to the control group (383.5 min ± 60.2min). It is concluded that the gastrointestinal transit of C57BL / 6 mice infected by 1500 larvae of Strongyloides venezuelensis presented changes recorded by the BAC around the peak of infection and at the end of the same.