Desinfestação de explantes de mogno africano (Khaya ivorensis a. Chev.) na propagação in vitro

Abstract

This study aimed to establish a disinfection protocol explants K. ivorensis, verify the feasibility of the use of side shoots as explants and determine the concentration and immersion time needed in NaClO solution for obtaining free explants of contaminants in MS medium. 3 experiments was performed using one and apical segments seedlings with 3 months of age and immersed in solutions containing 10, 20 and 30% NaClO, the immersion times of 10, 15, 20 and 25 minutes. Then it used the lateral shoots and soaking in 70% ethanol solutions containing 25 and 35% NaClO at the times 10 and 20 minutes and immersed in a fungicide Cuprogarb for 20 minutes. Finally, it was using explants of side shoots immersed in 70% ethanol, immersed in a solution containing 4 g L-1 systemic/ contact fungicidal action Cerconil for 5 minutes, and solutions containing 25% and 35% of sodium hypochlorite at times 20, 30 and 40 minutes. Endogenous contamination found in experiments only containing hypochlorite and also in 50% of explants used when the fungicide Cuprogarb. The explants disinfection K. ivorensis can be obtained using 70% ethanol 10 minutes fungicidal Cerconil 4 g.L-1 for 5 minutes immersion in NaClO solution at a concentration of 25% for 20 minutes. The treatment plant of donor arrays of explant is also of paramount importance to obtain high percentages of uncontaminated material.