Detecção de anticorpos anti-Brucella canis e anti-Brucella abortus em cães na cidade de Sinop, Mato Grosso, Brasil

Abstract

This study analyzed 93 non-castrated female dogs at variable ages and from different breeds and urban neighborhoods. The samples were collected during the annual antirabic vaccination campaign, on November 29th of 2015. Both risk factors related to the prevalence of the disease and antibody detection for canine brucellosis caused by Brucella canis and Brucella abortus were performed for this survey within the municipality of Sinop, Mato Grosso, Brazil. For the serologic analysis of Brucella canis, it was utilized the technique of Agar gel imunodiffusion (AGID) with bacterial (Brucella ovis) antigens and soluble lipopolysaccharides extracted from the sample Reo 198, from which the prevalence was 11.82%. The trial using agglutination test with AAT for Brucella abortus revealed 9.67% of positive cases. Although, this research has not indicated any correlation between the serologic results and the presence of risk factors, it is imperative to mention that the actual ratio may be higher than those estimated when considering that until the experiment is finished the negative samples had not been completely confirmed as negative.