Scrapie em um ovino no estado de Mato Grosso : relato de caso

Abstract

The supervised internship was held at the Veterinary Pathology Laboratory (PVL) Veterinary Hospital (HOVET) of the Federal University of Mato Grosso (UFMT) Campus Cuiabá where routine laboratory activities were conducting biopsy and cytology necropsy examinations, preparation and fixing materials to be embedded in paraffin and then cutting the tissue preparation, staining and mounting of histological slides. One of the cases studied in the PVL - Cuiabá, was described as a case report aimed to report the occurrence of Scrapie in the state of Mato Grosso, which is a Transmissible Spongiform Encephalopathy (TSE). In Veterinary Pathology Laboratory UFMT, Cuiabá, the necropsy of a sheep was performed, female, race suffolk 7 years. Fragments of tissues were collected, fixed in 10% formalin and routinely processed for histological examination. Microscopically, the soma nucleus and neuronal prolongations was noted vacuolization randomized to multifocal. Sections of the medulla of the óbex region were subjected to immunohistochemistry test, noting granular accumulation of material in perineuronal and perivascular space which is marked with anti-PrP antibody protein. Necropsy is a most important tool with systematic organ harvesting. It emphasizes the importance of refrigerated collection of CNS fragments to EET's differential diagnosis with other diseases with neurological signs such as endoparasitoses, hepatic encephalopathy, polioencephalomalacia, enterotoxaemias, diffuse suppurative meningitis, purulent encephalitis, rabies and herbivores other encephalopathies.