A influência do estresse ambiental no perfil lipídico e glicêmico de ratos Wistar tratados com prednisona

Abstract

The environment in which the individual is is directly related to quality of life. An inappropriate environment can lead to stress, in this situation there is the release of cortisol causing several effects in the body. Prednisone is a synthetic glucocorticoid, widely used in clinical practice once it has anti-inflammatory and immunosuppressive action, but depending on the time of treatment and dose used, it can cause changes in lipid metabolism and hyperglycemia. Thus, the objective of this study was to evaluate the influence of the stressful environment (metabolic cage) on the lipid and glycemic profile of Wistar rats treated with prednisone. For this, male Wistar rats were divided into groups: Cage with Prednisone (CP, n=7) and Metabolic Cage with Prednisone (MCP, n=7), treated daily with prednisone dissolved in the SyrSpend® vehicle, at a dose of 5mg/Kg /day for 28 days. The leukogram was evaluated before the beginning of treatment (day 0) and last day (28). On the 23rd day, the Oral Glucose Tolerance Test (OGTT) was performed, as well as the Area Under the Curve (AUC) and the Glucose Rate per Minute (GRM). On the 26th day, the Insulin Tolerance Test (ITT) was performed, as well as the area under the TTI curve and the Glycemic Uptake Index (GUI). At the end of the treatment, the rats were anesthetized and killed for analysis of serum biochemical parameters. Data were analyzed statistically, with significance at p<0.05. Comparing to the beginning of treatment, on the 28th day the segmented neutrophils of the GP group was increased and the eosinophils of the CP group decreased. In OGTT, the glycemic value of the GP group at 30' was higher than the CP group. As in the AUC of the OGTT and its TGM, it was verified that the glycemia of the GP rats was higher in the 30-60' time interval. Regarding serum lipids, there were no differences between groups; however, the total cholesterol and triglycerides values of both groups were found to be elevated to the reference values. It is concluded that the treatment with prednisone associated with stress interfered in the metabolism of glucose and lipids, as well as in the leukocyte profile.