Correlação da viscosidade com atividade tumoricida e tumoristática do extrato bruto de dioscoria bulbifera em células tumorais da mama

Abstract

Herbal medicines have been used in many parts of the world for thousands of years to treat illnesses, making them one of the safest and most easily accessible sources for treating various diseases, including cancer. In Brazil, plants that have a therapeutic effect end up being used by a large part of the population. In search of new discoveries for the inhibition or treatment of breast cancer, Dioscorea bulbifera, belonging to the Dioscoreaceae family, which originates from Asia and Africa, its bulbs can be edible or toxic, the physical characteristics of the species , is popularly known by several names such as Chinese yam, air yam and aerial potato. Despite the studies carried out in the medical and scientific environment, cancer still does not have efficient control. Breast cancer has different structures, clinical behavior and biological characteristics, being more common in women, it can be defined as invasive or non-invasive, and may undergo several variations depending on its stages. For the recommended treatment and chemotherapy, radiotherapy, endocrine therapies and surgeries, in an attempt to improve symptoms, alternatives are sought in phytotherapeutic treatments. The objective of this work was to evaluate blood viscosity in the presence of D. bulbifera extract, in addition to verifying the cell viability of mammary adenocarcinoma cells (MCF-7) in the presence of D. bulbifera extract at three different concentrations and at three times. incubation periods of 2, 8 and 12 hours. For this, 10 samples of peripheral blood were used for the analysis of blood viscosity. The MCF7- cells were cultivated in a nutrient medium and subsequently incubated in an oven at 37° C - 5% CO2 for 2, 8 and 12 hours with the D. bulbifera extract to determine their viability in relation to the control of MCF-7 cells. . The viability index was verified through the counting of live or dead cells, in the dye Acridine Orange, by the fluorescence microscopy technique. For the rheological analysis, 600 µl of the extracts were used in Microliter (µl), Nanoliter (nl) and Picolitro (pl) solutions, and 600 µl of the extract as peripheral blood, at 37°C, through the Compact Rheomenter Modulator equipment. It was observed the tumoricidal activity of the extract of D. bulbifera together with the stimulus MCF-7, when compared to the control, in the concentrations nl and pl, in the time of 2 hours. At 8 hours, the concentrations nl and pl had a tumorstatic effect, where the extract of D. bulbifera lost its tumoricidal effect. Similarly, in cells incubated for 12 hours, at all concentrations, the extract incubated with MCF-7 cells lost its action time when compared to the control. In rheology, it was possible to observe that the infusion of D. bulbifera extract, at concentrations µl, nl and pl, interacted with the blood, but did not affect its blood viscosity. Although more studies are needed, it can be accepted that this association is an important therapeutic strategy in the fight against cancer.