Viabilidade de células de adenocarcinoma mamário humano (MCF-7) em diferentes concentrações do extrato de Strychnos pseudoquina

Abstract

Brazil has a great diversity of plant species, that are used as sourcer of medicinal plants, one of the species of traditional use in Brazil is Strychnos pseudoquina, popularly known as “quina do cerrado”, a native plant of the Brazilian cerrado, used mainly for the treatment of gastric disorders, Still more phytochemical research need to be developed so that its full therapeutic potential can be used to treat diseases. One of the diseases that most challenges science is cancer due to all the physiological changes it causes in cells and the high mortality rat. This work aims to evaluate the viability of MCF-7 cells from human mammary adenocarcinoma in different concentrations of Strychnos pseudoquina extract incubated time. For this, serial dilutions of 1:10 of the quinone ethanol extract were made, and concentrations of µg, ng and pg were obtained. Later, the MCF-7 cells were adjusted to a concentration of 1x105 in a Neubauer chamber. Subsequently, 18 falcon tube containing 500µl of tumor cells and 50µl of quinine extract at different concentrations were incubated in an oven at 37°C and 5% CO2 for 2 hours and 24 hours. After the incubation period, viability was determined by fluorescence microscopy, where green cells were considered (live), and orange cells were considered (dead). The results shwed that cell viability was reduced in the period of 2 hours in all concentrations of the extract. However, in 24 hours, an increase in cell viability was noticed in the concentrations of µg and ng. In the concentration of pg not, there was a significant difference with the control group. Therefore, it is concluded that the extract of S. pseudoquina obtains action on the MCF-7 cell depending on the exposure time and not on the concentration to which it was exposed since new research must be developed to verify the exposure time that the extract stops having an action on the reduction in cell viability.