Avaliação do perfil bioquímico de ratos Wistar submetidos a diferentes regimes de redução de Prednisona

Abstract

Prednisone is one of the drugs of the glucocorticoid class, which has properties to alter the metabolism of carbohydrates, lipids and proteins, deregulating a series of physiological, biochemical and immunological processes, and is easily obtainable by the population, since it does not require doctor's prescription. It should be noted that there is a shortage of prednisone withdrawal protocols and, as this is a widely used drug, it is necessary to understand the biological interference of these different withdrawal protocols. The aim of this study was to evaluate the biochemical effects resulting from prednisone treatment in different drug reduction protocols in Wistar rats. Four groups of Wistar rats were used, each group with a type of reduction treatment regimen, namely: the Prednisone Group (GP, N=7) which received Prednisone throughout the treatment, Withdrawal Group (GR, N= 7) who received Prednisone 5mg/Kg for 14 days, and from the 15th day the treatment was replaced by SyrSpend® (drug vehicle) until the 28th day. Withdrawal Group A (GRA, N=7) received Prednisone 5mg/Kg for 14 days and underwent dose reduction and then withdrawal, where, on the 15th day there was a dose reduction to 2.5mg/Kg and on the 21st day, replacement by SyrSpend®. And the Withdrawal Group B (GRB, N=7) received Prednisone 5mg/Kg for 14 days and that, like the GRA Group, suffered a dose reduction, however, from the 15th day, there was a 50% reduction in the dose of Prednisone every 3 days and on the 27th day SyrSpend® was replaced. Drug treatment consisted of a single daily administration. Leukogram was performed on days 0, 14 and 28 of treatment and on the last day, the rats were euthanized and then blood was collected for serum assessments of Total cholesterol, triglycerides, high-density lipoproteins (HDL), nonHDL cholesterol (n -HDL), Glucose, albumin, total proteins, aspartate aminotransferase (AST) and alanine aminotransferase (ALT), in addition to total and differential leukocyte counts and Pearson's correlation between total leukocytes and lymphocytes with biochemical biomarkers. Results were considered statistically significant when p<0.05. There were no differences in the values of leukogram counts and serum protein, lipid and glucose biochemicals. However, the GRB group showed an increase in serum ALT activity compared to all other groups; in addition, in all groups with reduction and withdrawal protocol (GR, GRA and GRB) AST activity values increased in relation to continuous treatment. In this way, it was possible to conclude that the treatment of Wistar rats submitted to different treatment regimes shows that there is still no difference between these protocols to find an ideal model of weaning from the drug, opening space for the development of new methodologies, especially in the study higher doses and extended treatment times.