Protocolo de quantificação de reticulócitos em cães anêmicos

Abstract

The reticulocyte count is important both for the classification of anemias and also for the monitoring of the erythropoetic activity of the bone marrow. In the present study we aimed to compare dilutions, bathing times and stains, and to verify the efficiency and practicality of the tests based on the classification of the anemias. Three to five mL of blood from 40 adult dogs were collected. The reticulocytes were prepared with incubation in a water bath at 37 ° C, shortly after two slides were prepared for each technique, one only air-dried and one stained with Romanowskytype dye (Panótico Rápido®). The percentage value of corrected reticulocytes and their classification in response to anemia were obtained. Data were expressed as mean ± standard error of the mean (SEM) and analyzed by Analysis of Variance (ANOVA) one-way and the Shapiro-Wilk normality test. The data were considered significant when p <0.05. Time of water bath and staining were not relevant, making the examination faster without damaging the diagnosis. There was also no significant difference in the classification of the response to anemia, which makes variations of techniques possible.