Avaliação da bioatividade da eCG em diferentes temperaturas de conservação do produto

Abstract

eCG (equine chorionic gonadotropin), as it is known commercially, is a glycoprotein hormone produced and secreted by the endometrial calyces of pregnant women, with bihormonal activity, FSH and LH, essential in reproductive programs. The objective was to evaluate its bioactivity at different storage temperatures, detailing via transrectal palpation with an ultrasound device (US) the quantity and average size of follicles > 6mm produced from a superovulation protocol. Using 30 animals in 3 experimental groups with n=10 grouped by their weight, with treatment 01= negative control, acceptance of 5ml of intramuscular physiological solution (IM), treatment 02= positive control, acceptance of 1000UI of eCG IM at the recommended temperature 2°- 8°C and Treatment 03= 1000UI of IM eCG at room temperature for 7 days, both reconstituted at the time of application, resetting and synchronizing the emergence of the follicular wave on D0; applying treatments on D5 and performing ovarian assessment via US on D8. The results obtained by statistical analysis for the number of follicles > 6mm in T1 was 1.1 ± 1.19, considered inferior to T2 10.9 ± 4.22 and T3 11.8 ± 3.99 p<0.05, among which there was no statistical difference (p>0.05), demonstrating that there was similarity between the treatment responses. Repeatedly, the mean diameter of T1 (2.76 ± 2.14) was lower than the follicles of T2 (8.50 ± 0.72) and T3 (9.01 ± 0.84) (p<0.05), these no statistical difference (p>0.05). Concluding that eCG remained bioactive when administered via deep IM, after being stored at room temperature for 7 days.