Própolis de Tetragonisca angustula : atividade antifúngica sobre Colletotrichum lagenarium e indução de resistência em pepino

Abstract

Among the diseases in cultivated plants of common occurrence in agricultural systems, anthracnose in cucurbitaceous ones stands out, which the causal agent is the fungus Colletotrichum lagenarium. In the agronomic area, propolis has been investigated due to its antibiotic and antifungal properties. This work aimed to investigate the antifungal capacity and the potential of crude extract and hexane fraction of Tetragonisca angustula propolis to control and induce resistance of C. lagenarium, in cucumber. Crude propolis extract and hexane fraction of Tetragonisca angustula propolis were tested at concentrations of 0,1; 0,2; 0,3; 0,4 and 0,5 mg/mL; as well as Mancozeb® and distilled water as control. A protective assay of cucumber seedlings against C. lagenarium and two bioassays of antifungal activity were performed testing autoclaved and non-autoclaved treatments. In the seedling protection assay, the treatments of hexane fraction in concentrations of 0,1; 0,2; 0,3; 0,4 mg/mL and crude extract 0,2; 0,4; 0,5 mg/mL and the control were equal to Mancozeb® for disease severity. In the bioassay with non-autoclaved treatments, Mancozeb® was better than the others for Area Under Mycelial Growth Curve (AUMGC) and for growth rate. For the percentage of sporulation inhibition all non-autoclaved treatments were equal. In the bioassay with autoclaved treatments Mancozeb® was better for the AUMGC and equal to crude extract 0.3 mg/mL for growth rate. For percentage of sporulation inhibition, the crude extract 0.3 mg/mL and hexane fraction 0.1 and 0.2 mg/mL were equal to Mancozeb®.