Efeito do decocto de folhas de Morus nigra (amora) sobre o comportamento reológico do sangue periférico humano

Abstract

Medicinal plants have been a therapeutic alternative for thousands of years. In this scenario, the plant Morus nigra is presented, which is a species belonging to Morus, from the Moraceae family, known as blackberry or blackberry. This plant can be found in temperate and subtropical regions and can grow in a wide variety of climatic, topographic and soil conditions. Studies show that blackberry leaves are rich in flavonoids and phytohormones, which can modulate pathophysiological processes and prevent cardiovascular and metabolic diseases. Blood is a special type of connective tissue, fluid, made up of different cells dispersed in plasma, which circulates throughout the organism in a closed system. Understanding the rheology of human blood in the vascular network has been the subject of research at several institutions around the world due to its relevance for the diagnosis and treatments of various diseases that affect the human cardiovascular system. Therefore, the objective of this work was to evaluate the rheological behavior in human peripheral blood. When preparing the extract, the blackberry leaves were cut into smaller pieces to extract the components of the blackberry leaves. For the flow and viscosity curves, the parameters established were based on controlling the shear stress (T) from 0 to 5 Pa for the ascending curve and from 5 to 0 Pa for the descending curve. The results demonstrated that the proposed method for obtaining the decoction was efficient and it was also possible to formulate the established concentrations, being 100 µL.mL-1, 100 nL.mL1, 100 pL.mL-1. Rheological analyzes of flow and viscosity curves demonstrated that the behavior of blood in the presence and absence of stimuli and non-Newtonian with thixotropy and no concentration of the extract was able to change this behavior. However, the flow and viscosity curves of blood stimulated with blackberry extract at a concentration of 100 pL.mL1 increased the shear rate and decreased viscosity in relation to blood without stimuli. This result suggests the beginning of a modulatory effect from this dilution. We conclude that morus nigra extracts are hemorologically biocompatible for this tissue.