Determinação de intervalo de referência da enzima gama glutamiltransferase sérica em cães e gatos utilizando kits comerciais humanos

Abstract

Gamma glutamyltransferase (GGT) is an enzyme that is distributed across several tissues, concentrating its greatest activity in the pancreas and renal tubules, in turn, it is less active in the liver. Despite this, a large part of the GGT present in serum is derived from hepatocytes, being responsible for its elevation in blood in hepatobiliary injuries. Therefore, GGT is an important diagnostic tool in dog and cat clinics and it is necessary to standardize reagent kits in veterinary clinical laboratories. The present work aimed to determine reference ranges for serum GGT enzyme in domestic canines and felines using Wiener Lab® and Gold Analisa® brand reagents standardized for humans. In dogs, the measurements obtained were 1.8 ± 1.83 for Wiener Lab® reagent and a reference range of 0 to 5 U/L; 10.98 ± 4.04 for Gold Analisa® reagent and reference range from 2 to 17 U/L. In cats, the measurements obtained were 0.15 ± 0.48 for Wiener Lab® reagent and reference range 0 to 1 U/L; 8.63 ± 3.47 for Gold Analisa® reagent and reference range from 0 to 12 U/L. It was concluded that the reference ranges obtained in this experiment for serum GGT can be used for domestic canines and felines in laboratory routine, with the exception of the Wiener Lab® reagent for cats, as the results presented presented an extremely narrow value range (0 at 1 U/L), thus requiring further studies to assess its effectiveness.