Cladosporium cladosporioides isolado de gato com carcinoma de células escamosas : relato de caso

Abstract

This report describes the case of Cladosporium cladosporioides isolated from a periocular injury of a cat attended at the Veterinary Hospital. The injury was the main complaint of the owner, who had noticed it from about 4 months. Was reported an attempt of treatment of the lesion with anti-inflammatories and antibiotics, both without success. After a biopsy, the material was sent to the Microbiology and Molecular Biology Veterinary Laboratories and Veterinary Pathology Laboratory. The aim of this case report was to show the isolation of Cladosporium cladosporioides in a feline with squamous cell carcinoma. Case: The periocular injury of the feline was described by the owner as a scratch, with worsening of the injury with time. In history it was reported that the animal had access to the street, hunting habits and none of the other animals of the house had similar injury. The animal underwent to biopsy and cytology for a more accurate diagnosis of the case. For biopsy, the animal was sedated, an imprinting of the injury was made and a skin biopsy was taken using a punch needle. Further, a fragment was referred to the Veterinary Pathology Laboratory and one to Veterinary Microbiology and Molecular Biology Laboratories. At Microbiology Laboratory the material was seeded in Sabouraud agar and Sabouraud with chloramphenicol and incubated in temperatures of 25° and 30°. After about 10 days a colony with an olive color and velvety aspect was observed in cultive in both temperatures in Agar Sabouraud with and without Chloramphenicol. The macro and microscopic characteristics of the colony was consistent with Cladosporium cladosporioides. Subsequently the DNA was extracted and sequenced using oligonucleotides of Internal Transcript Spacer (ITS) 1 (5 'TCC GTA GGT GAA CCT GCG G 3') and ITS 4 (5 'TCC TCC TAT TGA TAT GC 3'). Histopathological analysis of the biopsy was consistent with squamous cell carcinoma and the cytology shown an acute inflammatory process. Discussion: The sequencing of the isolated confirmed Cladosporium cladosporioides. This genre known as saprophytic and commonly isolated from soy has been found in feline and canine´s fur [6, 13, 16], in conjuntive [19] and acoustic meatus of these animals [2]. In some cases it has been reported causing phaeohyphomycosis in the tissues of the animals and humans and even causing nodules in the nervous system of these, highlighting the importance of a better understanding of the action of this fungus in animals. The phaeohyphomycosis refers to cutaneous, subcutaneous and systemic fungal infections caused by Dematiaceous fungi, called in this way due to its characteristic dark for producing melanin in their mycelia. Histologically analyses with staining with hematoxylin-eosin (HE) and silver with Grocott technique, no fungal structure was observed, probably by a recent infection or difference of local, quality and characteristics of the segments of biopsy directed to the Pathology Laboratory and for Microbiology and Molecular Biology Laboratories, since the fragment was removed with a punch needle, constituting a physical limitation to the technic of being very small. Dematiaceous fungi can cause lesions in the skin, subcutaneous and systemic infestations of the animals and humans, and the scratch reported by owner may have been the gateway to the fungus in the subcutaneous tissue, where it was found. Tissue contamination hypothesis can be ruled out due to external decontamination of the sample before being seeded.