Efeitos da colostrinina resistina e melatonina sobre a viabilidade de células mononucleares do colostro de mães com excesso de peso. 2019. x, 35 f. TCC (Graduação em Biomedicina) - Universidade Federal do Mato Grosso, Campus Universitário do Araguaia, Instituto de Ciências Biológicas e da Saúde, Barra do Garças, 2019.

Abstract

O besity leads to an important chronic low-grade inflammation, causing damage to organs and tissues. Overweight is linked complications before and during gestation, leading to a series of health risks of the concept. During the first months of life of the newborn, breastmilk is the offering several advantages. Objective: the objective of the present study was was to evaluate the effects of colostrinin, resistin and melatonin on cell viability mononuclear cells from obese mothers. Materials and methods: 15 mothers, (n = 5), overweight (n = 5) and obesity (n = 5). The samples of colostrum with a volume of approximately 8 ml. Colostrum was centrifuged for 10 minutes at 160 G on 4C ° cooling, afterwards the counts on the Newbauer camera. Cell viability was assessed by the fluorescence microscopy with acridinaorange.Results: The present study overweight and obesity have not been shown to lead to changes in cell viability colostrum. It has been observed that colostrinin presents satisfactory results cell viability, with percentages higher than 95%. The same was verified for the resistance and melatoninane, which showed good results in terms of viability Conclusion: We concluded that colostrinin, resistin, and melatonin viability of more than 95%, which supports its applications in further studies to assessment of possible therapeutic activities in human colostrum.