Dano oxidativo no DNA de linfócitos de indivíduos com risco cardiovascular com e sem excesso de peso

Abstract

The high prevalence rate and the increase in the occurrence of deaths due to Cardiovascular Disease (CVD) have been related to the frequency of modifiable risk factors present in the population. Among the risk factors, excess weight is associated with increased oxidative damage in the DNA and may promote the development of CVDs. The objective of this work was to evaluate the oxidative DNA damage of individuals with cardiovascular risk (CVR) with excess weight (> 25 kg / m2) and without. The lifestyle and anthropometric data of 40 individuals of both sexes, aged between 20 and 59 years, with some risk factors for cardiovascular disease were analyted. The basal oxidative damage in the DNA was determined by the comet assay modified with the enzyme DNA-formamidopyrimidine glycosylase (FPG). The mean age was 41 ± 9.2 years, with 50% (n = 20) males and 50% (n = 20) females, with a mean BMI of 30.88 ± 3.55 kg /m2, 80% (n = 32) with excess weight (BMI ≥25kg /m ²) and 20% (n = 8) without excess weight, presenting waist ratio of 0.87 ± 0.07 and 0.7 ± 0,09, respectively. We found a higher frequency of DNA oxidative damage in the overweight group (15.09%) when compared to the non-overweight group (11.02%), but with no difference (p = 0.685). Therefore, it was concluded that despite the high prevalence of overweight and obesity in the studied sample, there were no significant differences in oxidative DNA damage in the groups with and without overweight with RCV.