Avaliação da viabilidade celular de adenocarcinoma mamário humano (MCF-7) incubado na presença do extrato de babaçu (Orbignya martiniana) em três diferentes concentrações

Abstract

The plant species Orbygnia martiana, known as babassu, has been used in folk medicine in the treatment of various inflammatory diseases, and among this disease cancer, medicinal plants have always been objects of studies in an attempt to find new sources of active principles. On the other hand, human breast cancer is one of the biggest causes of death among women and the search for effective treatments still represents a great challenge. That said, this study aims to verify the cell viability of human mammary adenocarcinoma (MCF-7) cell lines in the presence of babassu mesocarp extract at three different concentrations picogram, nanogram and microgram incubated at two different times: 2 hours and 24 hours . For this, three different concentrations of babassu extract were used, picogram, nanogram and microgram, which underwent two incubation times, 2 and 24 hours. After incubation, MCF-7 cells were used in the presence of babassu extract, cell viability was determined by the acridine orange aeration method and determination by fluorescence microscopy. The data obtained were treated statistically (specify the type of statistics used). Noting that in the time of 2 hours of incubation it presented a viability of 81.42%. While the group containing nanogram concentration presented 82.28% viability and the picogram concentration group presented the lowest viability index, accounting for 78.97% cell viability when compared to the control group. In the 24-hour incubation time, the mean values of cell viability were 81.42% in the microgram concentration, 82.23% in the nanogram concentration and 77.79% in the picogram concentration. In the 2-hour incubation period, babassu changes cell growth, making it possible to use it in the treatment of breast cancer.