Efeito do óleo da semente de abóbora sobre o trânsito gastrointestinal de camundongos

Abstract

The use of vegetable oils has increased, due to nutritional aspects and beneficial properties to the body when consumed properly. Pumpkin seed has a high content of fat, protein, mineral and bioactive substances, characteristics that can be useful for anthelmintic purposes and is capable of removing free radicals. The objective was to quantify the total carotenoids of pumpkin seed oil and the gastrointestinal transit time in rodents treated with the oil. The commercial and cold-pressed vegetable oil (Pazze®) was read in a spectrophotometer at 450 nm for the determination of total carotenoids. at the Food Analysis Laboratory at UFMT. For in vivo analyses, 13 adult male BALB/c mice were used, the animals were placed in the Reproductive Physiology and Toxicology Laboratory (Physiotox) with water and food ad libitum. 0.9% saline solution by gavage for 15 days and Oil (N=5) treated with 4mL/kg pumpkin seed oil by gavage for 15 days. Gastrointestinal transit time was determined by Alternating Current Biosusceptometry (BAC), where animals were fasted for 12 hours and then 250g of magnetically labeled feed were ingested. Every 20 minutes for a period of 10 hours, the fecal pellets were collected and the magnetic intensity determined to calculate the fecal elimination rate. ±22.00 and oil group 389.30 ± 24.48**, on the 14th day the control group 414.89 ± 32.60 and the oil group 376.69 ± 13.55** demonstrating that the group treated with oil, intestinal transit time was accelerated. In conclusion, the pumpkin seed has the presence of bioactive compounds that help in the maintenance of the organism with antioxidant and in the analysis of the fecal elimination rate, with the oil group in relation to the control group, which may interfere with the nutrition of nutrients and drugs.