Efeitos da serotonina adsorvida a microesferas de polietilenoglicol sobre a viabilidade celular

Abstract

Serotonin, a hormone produced in our body, is responsible for playing roles in regulating various behavioral and biological functions in our body, inducing metabolic effects in various cell types, affecting the immune system, central nervous system and gastrointestinal system. Polyethylene glycol is widely used in various pharmaceutical formulations due to its chemical properties, such as high solubility in water and organic solvents, biocompatibility, non-toxicity and non-immunogenicity. This material has been used for the production of microparticles that can incorporate substances such as drugs, peptides and hormones, and has several advantages, including the ability to encapsulate multiple molecules and effectiveness in modified release. Given the above, the present study aimed to evaluate the cellular viability of serotonin adsorbed on polyethylene glycol (PEG) microspheres. PEG microspheres were prepared in a process involving heat and subsequently adsorbed with serotonin, the cell viability test was performed using peripheral blood mononuclear cells (PBMC) and were divided into 4 experimental groups, namely: control group with cells only , serotonin group, PEG and PEG/serotonin group. The effects of PEG microspheres adsorbed with serotonin on cell viability were determined. The presence of serotonin reduced cell viability, however, when adsorbed onto PEG microspheres, no change in cell viability was observed. The results obtained suggest that PEG microspheres possibly reduce the effects caused by serotonin on cell viability.