Extração, fracionamento e isolamento de resibufogenina a partir do veneno de Rhinella marina por CLAE

Abstract

The venoms of bufonids, a family of frogs, are home to a variety of bioactive substances, known as bufadienolides, which are chemical compounds recognized for their wide-ranging pharmacological properties, such as cardiotonic, anti-tumour and anti-inflammatory activity. These substances act as inhibitors of the Na+/K+ ATPase pump, resulting in changes in the intracellular concentration of sodium and calcium, with a direct impact on cardiac and vascular function. The Rhinella marina species (R. marina), also known as the "cururu frog" or "bullfrog", is a frog widely distributed in South America. Its venom contains bufadienolides, including Resibufogenin (RBG), a compound of great interest due to its potential applications. To isolate the RBG present in R. marina venom, we used the analytical technique of High Performance Liquid Chromatography (HPLC), a powerful tool for separating, identifying, isolating and purifying compounds in complex samples, such as toad venoms. This technique is based on the differentiated displacement of the compounds in a mixture through a stationary phase, promoted by a mobile phase through a chromatographic column, allowing purified substances to be obtained. Throughout the research, the isolation of RBG with a purity of 100% was successfully achieved. This study plays an important role in science, as it contributes to understanding the pharmacological properties of the bufadienolides present in R. marina venom. It also opens up new perspectives for future research into the therapeutic potential of this compound, particularly in relation to heart disease and other conditions. The RBG isolated in this study can be subjected to biological activity tests, which are essential for assessing its impact on biological systems and for identifying possible therapeutic applications, as well as understanding its effects on cardiac and vascular function. This research represents a significant advance in science, with promising implications for scientific research in the chemical and pharmacological fields.