Microscopyscanner – um kit desenvolvido com arduino, impressão 3d e processamento digital de imagens para escanear e construir um panorama de imagem de microscopia

Abstract

Used to view tiny structures, the microscope is an essential equipment for any laboratory, however it is observed that the users of this laboratory equipment suffer from discomforts and are also subject to the development health problems occasioned by their prolonged use. Faced with this, equipment for scanning microscopy slides have been developed and are sold, however they are expensive. Some works have sought to remedy such a need by creating low cost systems using 3D printing, Arduino to control the microscope and webcam or cameras adapted to the microscopes to capture the images. Faced with this, this paper proposes the development of a kit called "MicroscopyScanner" to capture images generated by a microscope, sweeping the slide through an equipment developed with Arduino technology and 3D printing, and digitally processing the images to generate a single image. The system consists of two softwares, a mobile application for Android platform developed with the framework Lazarus Android Mobile Wizard, responsible for capturing the images in the eyepiece of the microscope and to control in a semi-automatic, or automatic, way the microscope table and a computer software, that realizes the panoramic construction of images, from sample of slides, that uses methods of digital processing of images of the library OpenCV. With the system developed, the user can observe the sample of the screen of the cell phone, command the movement of the blade in the X and Y directions, capture images traversing a specified area and construct the panoramic image of the sample. The Handling the microscope with the help of this system becomes a less tedious task, in which, instead of analyzing the sample through the eyepiece of the microscope and performing the manual movement of the slide, a panorama can be recorded and constructed, automatically, of the region of interest, which allows the subsequent observation of the sample on digital media on a computer screen, or also use it in some software for diagnostic aid through images. Another attractive feature of this system is its low acquisition cost, priced at around $ 450, at the time of completion of this work, whereas professional blade scanners cost more than $ 53,900.