Produção de Beauveria bassiana em meio sólido

Abstract

The objective of this work was to evaluate the large-scale production of Beauveria bassiana, in terms of quantity and quality. The work was established and carried out at the company On Farm Bio Insumos de Sinop, which produces entomopathogenic fungi for agriculture. The entire production process, from the matrix to the finished product, took 16 days of production. Were produced Beauveria matrices b. in the rice culture medium over a period of 9 days, for growth and development, in an isolated and sterilized production room. B. bassiana was evaluated in 4 different treatments with a variation of 2 positions. (P1 – Packages vertically and P2 – Packages horizontally). The treatments were divided by weight, T1 with 350g, T2 with 500g, T3 with 700g and T4 with 1000g, with the packages being randomly placed on the shelves and inoculated in the proportion of 10ml (T1), 20ml (T2), 30ml (T3) and 40ml (T4) respectively, varying with the weight of the treatments. For the production process, rice culture medium was used for the development and growth of the fungus B. bassiana The growth and development took place in 7 days inside polypropylene packages, in a room with a 12-hour photoperiod and a controlled temperature maintained at an average of 25°C. The evaluations performed were spore count in Neubauer chamber and viable spore count (germination). The best result obtained, both for spore count and for germination, was for the T1P2 treatment (350g - Horizontal).