Estudo histomorfométrico de folículos ovarianos em feto bovino

Abstract

This study aims to characterize and quantify the fetal ovarian population, describe the morphological parameters of ovarian follicular development, and analyze the effect of hemorrhagic cysts on the development of ovarian follicles in bovine fetuses. For this purpose, the ovaries of 10 Nellore cattle ferns (Bos primigenius indicus), with ages ranging from 6, 7 and 9 months, from pregnant cows slaughtered in slaughterhouses, were used. The ovaries were dissected, weighed on an analytical balance with an accuracy of 0.05g and measured with the aid of a caliper with an accuracy of 0.05 mm. Then, they were separated into right and left and preserved in 10% formaldehyde. The samples were processed and embedded in paraffin, being sectioned into longitudinal sections of 3μm thickness and stained by the hematoxylin-eosin method. The results revealed a population of follicles at different stages of development, with variation in follicular types and amounts. The presence of hematocysts in the cortical region was observed in 7 of the 20 ovaries analyzed, as well as the presence of tertiary follicles in all fetal ages analyzed. Regarding the analysis of follicular diameter, a mean value for primary follicles of 42.3 μm in the right ovary and 40.5 μm in the left ovary was found. The primary follicular circumference showed a mean of 128.6 ± 131.3 μm in this study. The influence of hematocysts on the follicular population, as well as on the diameter and perimeter of all follicular types, was observed, suggesting that the presence of these structures may affect the normal development of follicles. This study highlights the need for future investigations for a more in-depth understanding of these changes and their impact on fetal development and reproductive health.