Investigação molecular para enteropatógenos virais causadores de gastroenterite aguda em cães atendidos no Hospital Veterinário da Universidade Federal de Mato Grosso

Abstract

Viral gastroenteritis represents a frequent occurrence in the practice of clinical veterinary medicine, primarily affecting young animals less than one year of age. Coinfection by viral agents has been widely documented in several studies conducted in different regions of the world. Co-infection by viral agents has been widely documented in several studies conducted in different regions of the world. The main agents associated with such cases include canine parvovirus (CPV), canine circovirus (CaCV) and canine adenovirus (CAdV-1). In Brazil, canine parvovirus was initially detected in the 1980s, in the state of São Paulo, and there are currently records of three variants in circulation: CPV-2a, CPV-2b, CPV-2c. Canine Circovirus, another significant viral agent in acute gastroenteritis, was identified in the United States in 2012; in Brazil, reports of cases involving this agent are scarce. Canine Adenovirus, identified since 1947, comprises 2 serotypes, one of which is the serotype responsible for infectious canine hepatitis. For improved diagnosis of gastroenteritis cases, in addition to detailed anamnesis, it is essential to carry out a thorough physical examination and laboratory diagnostic support. This study aimed to identify the presence of viral enteropathogens, such as Canine Parvovirus, Canine Circovirus and Canine Adenovirus, in dogs with acute gastroenteritis treated at the Veterinary Hospital of the Federal University of Mato Grosso (HOVET-UFMT). A total of 54 samples from animals with acute gastroenteritis were examined, as well as a sample from the Veterinary Pathology department. Samples were extracted according to the manufacturer's instructions using the MagMax CORE Nucleic Purification – Applied Biosystems – Thermo Fisher Scientific extraction kit. The molecular diagnosis was based on studies conducted by Streck (2013), Dowgier (2016) and Li (2013), using specific primers. Of the 54 animals included in the study, 28 were males and 26 were females, with varying ages. Using the Real-Time Polymerase Chain Reaction (qPCR) technique, canine Parvovirus was detected in 85.18% of the animals, canine Adenovirus in 1.85%, while canine Circovirus was not detected in any sample.