Repercussões maternas do tratamento de curatella americana l. durante a prenhez de ratas não-diabéticas e com diabete moderado

Abstract

INTRODUCTION: During pregnancy, hyperglycemia can cause maternal and fetal complications, leading to reproductive changes. In this way, alternatives are sought to treat diabetes and prevent its complications. One such possibility is the use of medicinal plants, such as the American Curatella, known as trash. However, there is no scientific evidence of its hypoglycemic effect and the safety of its use during pregnancy. OBJECTIVE: To evaluate the effects of Curatella americana treatment on maternal parameters of non-diabetic and diabetic pregnant rats. METHODOLOGY: Wistar rats, 90 days old, were mated to obtain newborns. Diabetes was induced in females 24 hours after birth by subcutaneous injection of Streptozotocin at a dose of 100 mg / kg. In adulthood (110 days), oral glucose tolerance test (OGTT) was performed to confirm the diabetes model with moderate intensity hyperglycemia. After confirmation of diabetes, the rats were mated and distributed in 4 experimental groups (n = 12 animals / group): Control: Non-diabetic rats treated with water; Control Treated: Non-diabetic rats treated with the plant extract; Diabetic: Diabetic rats treated with water; Diabetic Treated: Diabetic rats treated with the plant extract. Administration of the aqueous extract of Curatella americana at the dose of 300 mg / kg was performed daily orally (gavage) throughout pregnancy. Weight, water intake, food intake and blood glucose levels were evaluated weekly. On the morning of the 21st day of pregnancy, the rats were anesthetized and killed, and the heart, liver, spleen, pancreas and kidneys were removed to be weighed. The uterus was removed and the numbers of luteal bodies, implantation sites, resorptions, live and dead fetuses were counted to calculate pre and post-implantation loss rates. RESULTS: The maternal glycemia of the rats of the diabetic groups was higher than that of the non-diabetic rats at day 0 of pregnancy. Treatment with the plant did not alter the glycemic levels of non-diabetic animals, but promoted an increase in glycemia on day 14 of pregnancy in diabetic animals. There was no difference in body weight, dietary intake, water intake and body weight among experimental groups. Both diabetic groups exhibited higher pre-implantation loss rates and lower maternal weight gain. CONCLUSION: Treatment with aqueous extract of Curatella americana at the dose tested had no hypoglycemic effect in pregnant rats and the use of the plant did not cause maternal injury during the pregnancy of the rats.